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UPDATE
2024-12-12
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National Stem Cell Bank

National Stem Cell Bank (NSCB) is a biorepository of the human pluripotent stem cell lines. To be the world`s leading class as an infrastructure supporting regenerative medicine, the bank provides the high quality stem cells and related information for regenerative medicine. The bank will compliance with standards of bioethics and safety regulations.

Process

Researcher

  • Application form for deposition

National Stem Cell Bank

  • Receives the application form
  • Receives the stem cells
  • Analyses the basic characteristics
  • Review of steering committee
  • Exchange of deposit contract

Steering Committee

  • Oversees bank activities

Cell lines available from the bank

Collection of Pluripotent Stem Cells

Collection of Pluripotent Stem Cells - Type, Number, Note
Type Number Note
hESC 19
hiPSC 106
  • Healthy donor 12 lines
  • HLA-homozygotes 23 lines
  • Disease-specific 42 lines
  • Fluorescent protein tagged 10 lines
  • Gene-edited 12 lines
  • Parthenogenic 3 lines

Cell lines available from the bank

Collection of Pluripotent Stem Cells - Type, Cell Name, Note
Cell type Cell line Provider Origin and establishment method Culture condition QC data NGS
data
Reference
hESC SNUhES3 Seoul National University ㆍOrigin: human embryo
ㆍRegistry Number : hES12010005
ㆍFeeder (STO)
ㆍhPSC media
PDFdownload
SNUhES4 Seoul National University ㆍOrigin: human embryo
ㆍRegistry Number : hES12010006
ㆍFeeder (STO)
ㆍhPSC media
PDFdownload
SNUhES31 Seoul National University ㆍOrigin: human embryo
ㆍRegistry Number : hES12010037
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CHA-hES15 CHA University ㆍOrigin: human embryo
ㆍRegistry Number : hES12010028
ㆍFeeder (STO)
ㆍhPSC media
PDFdownload
hiPSC
(healty donor cell-derived)
hFmiPS1 KNIH ㆍParental cell : Dermal fibroblast
ㆍReprogram : modified RNA
ㆍAbnormal karyotype
ㆍFeeder (STO)
ㆍhPSC media
PDFdownload
hFmiPS2 KNIH ㆍParental cell : Dermal fibroblast
ㆍReprogram : modified RNA
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hFSiPS3-1 KNIH ㆍParental cell : Dermal fibroblast
ㆍReprogram : Sendai virus
ㆍhFSiPS1 sub-line
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hUSiPS2 KNIH ㆍParental cell : Urinal cell
ㆍReprogram : Sendai virus
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hAdMSiPS1 KNIH ㆍParental cell : Adipocyte-MSC, TALEN
ㆍReprogram : Sendai virus
ㆍFeeder (STO)
ㆍhPSC media
PDFdownload
CMC-hiPSC-003 The Catholic University of Korea ㆍParental cell : Bone marrow
ㆍReprogram : Sendai virus
ㆍHLA-homozygote
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CMC-hiPSC-005 The Catholic University of Korea ㆍParental cell : Cord blood
ㆍReprogram : Sendai virus
ㆍHLA-homozygote
ㆍAbnormal karyotype
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CMC-hiPSC-009 The Catholic University of Korea ㆍParental cell : Cord blood
ㆍReprogram : Sendai virus
ㆍHLA-homozygote
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CMC-hiPSC-011 The Catholic University of Korea ㆍParental cell : Cord blood
ㆍReprogram : Sendai virus
ㆍHLA-homozygote
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CMC-hiPSC-022 The Catholic University of Korea ㆍParental cell : Cord blood
ㆍReprogram : Sendai virus
ㆍHLA-homozygote
ㆍFeeder-free
ㆍVN-E8
PDFdownload
NU01-EiPS07 KNIH ㆍParental cell : Urinal cell
ㆍReprogram : Episomal vector
ㆍFeeder-free
ㆍVN-E8
PDFdownload
PB01-EiPS21 KNIH ㆍParental cell : Peripheral blood cell
ㆍReprogram : Episomal vector
ㆍFeeder-free
ㆍVN-E8
PDFdownload
Disease
-Specific
hiPSC
DKH005i-A KNIH ㆍParental cell : Peripheral blood cell
ㆍReprogram : Sendai virus
ㆍDisease : Eye disease, Senior Loken syndrome
ㆍFeeder-free
ㆍVN-E8
PDFdownload
DKH090i-A KNIH ㆍParental cell : Peripheral blood
ㆍReprogram : Sendai virus
ㆍDisease : Eye disease, Leber congenital amaurosis
ㆍFeeder-free
ㆍVN-E8
PDFdownload
LQT01-hiPSC KNIH ㆍParental cell : Peripheral blood
ㆍReprogram : Sendai virus
ㆍDisease : Heart-signaling disorder, long QT syndrome type 2, KCNH2 (c.453delC)
ㆍFeeder-free
ㆍVN-E8
PDFdownload
LQT08-hiPSC KNIH ㆍParental cell : Peripheral blood
ㆍReprogram : Sendai virus
ㆍDisease : Heart-signaling disorder, long QT syndrome type 1, Brugada syndrome, KCNQ1 (c.569G>A)
ㆍFeeder-free
ㆍVN-E8
PDFdownload
Fluorescent
protein-tagged hiPSC
NCRM5AS1-iCAGcGFP.c9 NIH-CRM
(U.S)
ㆍGenetic modification (GFP), TALEN
ㆍParental cell : Peripheral blood (CD34+)
ㆍReprogram : Episomal vector
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CMC-003i-Nestin.EGFP KNIH ㆍGenetic modification (EGFP in Nestin gene), CRISPR/CAS9
ㆍParental cell : CMC-hiPSC-003
ㆍFeeder-free
ㆍVN-E8
PDFdownload
CMC-003i-Pdx1.EGFP KNIH ㆍGenetic modification (EGFP in PDX1 gene), CRISPR/CAS9
ㆍParental cell : CMC-hiPSC-003
ㆍFeeder-free
ㆍVN-E8
PDFdownload
PB01-EiPS21
(GFP-NLS7)
KNIH ㆍGenetic modification (EGFP-NLS in AAVS1 site), CRISPR/CAS9
ㆍParental cell : PB01-EiPS21
ㆍFeeder-free
ㆍStemFlex
PDFdownload
PB01-EiPS21(Ruby2) KNIH ㆍGenetic modification (Ruby in AAVS1 site), CRISPR/CAS9
ㆍParental cell : PB01-EiPS21
ㆍFeeder-free
ㆍStemFlex
PDFdownload
PB01-EiPS21-EGFP04 KNIH ㆍGenetic modification (EGFP in AAVS1 site), CRISPR/CAS9
ㆍParental cell : PB01-EiPS21
ㆍFeeder-free
ㆍVN-E8
PDFdownload
PB01-EiPS21-EGFP05 KNIH ㆍGenetic modification (EGFP in AAVS1 site), CRISPR/CAS9
ㆍParental cell : PB01-EiPS21
ㆍFeeder-free
ㆍVN-E8
PDFdownload
Gene-Edited hiPSC hiPSC-TLR2KO KNIH ㆍGenetic modification (TLR2 knock-out), CRISPR/CAS9
ㆍ(parental cell) modified from hiPSC CMC-hiPSC-003
ㆍParental cell : CMC-hiPSC-003
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hiPSC-TLR3KO-A49 KNIH ㆍGenetic modification (TLR3 knock-out), CRISPER/CAS9
ㆍParental cell : CMC-hiPSC-003
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hiPSC-TLR7KO-A59 KNIH ㆍGenetic modification (TLR7 knock-out), CRISPER/CAS9
ㆍParental cell : CMC-hiPSC-003
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hiPSC-TLR8KO-A10 KNIH ㆍGenetic modification (TLR8 knock-out), CRISPER/CAS9
ㆍParental cell : CMC-hiPSC-003
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hFSiPS1_DUSP6KO KNIH ㆍGenetic modification (DUSP6 knock-out), CRISPR/CAS9
ㆍParental cell : hFSiPS1
ㆍFeeder-free
ㆍVN-E8
PDFdownload
hFSiPS1_DUSP6KOA KNIH ㆍGenetic modification (DUSP6 knock-out), CRISPR/CAS9
ㆍParental cell : hFSiPS1
hFSiPS1_DUSP6KO subline
ㆍFeeder-free
ㆍVN-E8
PDFdownload

Deposition Information

Please contact the National Stem Cell Bank (NSCB)

  • e-mail : nscb@korea.kr
  • Phone : +82-43-249-2518

KSCB International Collaboration

Collaborations

Related organization & society

The GMP Manufacturing facility

The GMP Manufacturing facility which is belonged to the National Center for Stem Cell and Regerative Medicine (NCSR) was opened in 2016 to accelerate clinical research of the stem cell and regenerative medicine field. In addition to manufacturing and distribution of iPSC sources that can be used for clinical research, we support manufacture of cell therapeutics for clinical research. Recently, by starting a GMP training course for manufacture of advanced regenerative therapeutics, we expect that the GMP market will be facilitated.

Process

  • GMP Manufacturing and Supply of GMP Grade hiPCS Cell lines
  • Contract Manufacturing Service of Cell Therapeutics for Clinical Research
  • Contract Quality Test Service of of Cell Therapeutics for Clinical Research

Detailed Service

  • GMP Manufacturing of Cell Therapeutics for Clinical Research
  • GMP Quality Test of Cell Therapeutics for Clinical Research
  • MCB/WCB Banking of Cell Therapeutics for Clinical Research
  • GMP Training
  • GMP Consulting

GMP Manufacturing Facilities

  • Total area : 5,181㎡ (5 story building)
  • GMP area : 1,620㎡ (3rd floor)
GMP Manufacturing Facilities - Floors, Cell Name
Floors Cell Name
5 AHUs
4 Maintenance Floor
3 Production, QC Lab., Warehouse
2 Cell Bank, R&D Lab., Office
1 Meeting room, BMS room
B1 Mechanical, Electrical

GMP Manufacturing Facilities

Procedure for GMP Manufacturing Service

  • Researchers who plan for clinical study for cell therapeutics apply for the manufacturing service
  • Committee of GMP manufacturing facility reviews the plan
  • If the plan is accepted, researcher and NCSR makes contract for the manufacturing
  • After review and transfer of manufacturing process, NCSR progresses the process validation and manufacturing

Requirements to Apply

  • Verified data for safety and efficacy of products at GLP facilities
  • The Completed GMP manufacturing process and analytical methods
  • Period of manufacturing processes not exceeding nine (9) months
  • Preparedness to start clinical study within one year after finishing manufacturing of products

Steps to Apply

  1. 1

    Interview
    By e-mailing,
    Calling or visiting
  2. 2

    Pre-review
    Review of utilization plan
    (Draft)
  3. 3

    Review and Approval
    Registration and Evaluation
    of Utilization Plan
    (By Review Board Members)
  4. 4

    Contract
    Contract signing and
    provision of GMP Service
    (Post-monitoring of service goals)

Criteria of Approval

  • Suitability of Utilization Plan
  • Competency of Processing Techniques
  • Possibility of Practical Application
  • Relevance of Outcome Utilization
  • Ripple Effect of Regenerative Medicine

Major GMP Equipment

Major GMP Equipment - Division, Species, Amounts
Division Species Amounts
Utility HVAC System, Gas System, BMS, TMS, PMS, LN2 System, etc. 45
Process BSC, CO2 Incubator, Centrifuge, RP Microscope, CRF, etc. 104
QC Fluorescent Microscope, FACS, Endotoxin Analyser, RT-PCR, Cytometer, NAE, Air Particle Counter, Air Sampler, etc. 71
Storage LN2 Tank, Cell Storage Tank, Deep Freezer, Refrigerator, Freezer, etc. 23
Total 243

Time to Apply

Anytime throughout the year
Please contact the National Center for Stem Cell and Regenerative Medicine (NCSR)

  • e-mail : chy0986@korea.kr
  • Phone : +82-43-249-2522
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