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Microorganisms, 2020.8(9), 1-19, DOI: https://doi.org/10.3390/microorganisms8091434
Resistance profiling and molecular characterization of extended-spectrum/plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from healthy broiler chickens in South Korea
Hyun-Ju Song, Dong Chan Moon; Abraham Fikru Mechesso; Hee Young Kang; Mi-Hyun Kim; Ji-Hyun Choi; Su-Jeong Kim; Soon-Seek Yoon and Suk-Kyung Lim
We aimed to identify and characterize extended-spectrum -lactamase (ESBL)-and/or plasmid-mediated AmpC -lactamase (pAmpC)-producing Escherichia coli isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 E. coli isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials. ESBL/pAmpC-production was noted in 14% (46/332) of the isolates. Six of the CTX-M- -lactamase-producing isolates were found to co-harbor at least one plasmid-mediated quinolone resistance gene. We observed the co-existence of blaCMY-2 and mcr-1 genes in the same isolate for the first time in Korea. Phylogenetic analysis demonstrated that the majority of blaCMY-2-carrying isolates belonged to subgroup D. Conjugation confirmed the transferability of blaCTX-M and blaCMY-2 genes, as well as non- -lactam resistance traits from 60.9% (28/46) of the ESBL/pAmpC-producing isolates to a recipient E. coli J53. The ISECP, IS903, and orf477 elements were detected in the upstream or downstream regions. The blaCTX-M and blaCMY-2 genes mainly belonged to the IncI1, IncHI2, and/or IncFII plasmids. Additionally, the majority of ESBL/pAmpC-producing isolates exhibited heterogeneous PFGE profiles. This study showed that healthy chickens act as reservoirs of ESBL/pAmpC-producing E. coli that can potentially be transmitted to humans.
- DOI: https://doi.org/10.3390/microorganisms8091434
- ISBN or ISSN: 2076-2607
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.