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Cell, 2020.181, 914-921, DOI: https://doi.org/https://doi.org/10.1016/j.cell.2020.04.011
The architecture of SARS-CoV-2 tranome
Dongwan Kim, Joo-Yeon Lee;Jeong-Sun Yang;Jun Won Kim;V. Narry Kim;Hyeshik Chang
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its tranomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 tranome and epitranome.DNA nanoball sequencing shows that the tranome is highly complex owing to numerous discontinuous tranion events. In addition to the canonical genomic and 9 subgenomic RNAs, SARSCoV-2 produces trans encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral trans, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 30 tail. Functional investigation of the unknown trans and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.
- DOI: https://doi.org/https://doi.org/10.1016/j.cell.2020.04.011
- ISBN or ISSN: 0092-8674
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.