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Emerging Microbes & infections, 2020.9, 2714-2726, DOI: https://doi.org/https://doi.org/10.1080/22221751.2020.1861914
An infectious cDNA clone of a growth attenuated Korean isolate of MERS coronavirus KNIH002 in clade B
Minwoo Kim, Hee Cho;Seung-Hoon Lee;Woo-Jung Park;Jeong-Min Kim;Jae-Su Moon;Geon-Woo Kim;Wooseong Lee;Hae-Gwang Jung;Jeong-Sun Yang;Jang-Hoon Choi;Joo- Yeon Lee;Sung Soon Kim;Jong-Won Oh
The MERS-CoV isolated during the 2015 nosocomial outbreak in Korea showed distinctive differences in mortality and transmission patterns compared to the prototype MERS-CoV EMC strain belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 in the clade B phylogenetically far from the EMC strain, and generated a recombinant MERS-CoV expressing red fluorescent protein. The virus rescued from the infectious clone and KNIH002 strain displayed growth attenuation compared to the EMC strain. Consecutive passages of the rescued virus rapidly generated various ORF5 variants, highlighting its genetic instability and calling for caution in the use of repeatedly passaged virus in pathogenesis studies and for evaluation of control measures against MERS-CoV. The infectious clone for the KNIH002 in contemporary epidemic clade B would be useful for better understanding of a functional link between molecular evolution and pathophysiology of MERSCoV by comparative studies with EMC strain.
- DOI: https://doi.org/https://doi.org/10.1080/22221751.2020.1861914
- ISBN or ISSN: 0000-0000
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.