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Scientific Reports, 2020.10, 16200-0, DOI: https://doi.org/https://doi.org/10.1038/s41598-020-72879-7
Antiviral activity of digoxin and ouabain against SARS-CoV-2 infection and its implication for COVID-19
Junhyung Cho, Young Jae Lee; Je Hyoung Kim; Sang il Kim; Sung Soon Kim; Byeng-Sun Choi; Jang-Hoon Choi
The current coronavirus (COVID-19) pandemic is exacerbated by the absence of effective therapeutic agents. Notably, patients with COVID-19 and comorbidities such as hypertension and cardiac diseases have a higher mortality rate. An efficient strategy in response to this issue is repurposing drugs with antiviral activity for therapeutic effect. Digoxin (DIG) and ouabain (OUA) are FDA drugs for heart diseases that have antiviral activity against several coronaviruses. Thus, we aimed to assess antiviral activity of DIG and OUA against SARS-CoV-2 infection. The half-maximal inhibitory concentrations (IC50) of DIG and OUA were determined at a nanomolar concentration. Progeny virus titers of single-dose treatment of DIG, OUA and remdesivir were approximately 103-, 104- and 103-fold lower (> 99% inhibition), respectively, than that of non-treated control or chloroquine at 48 hours post-infection (hpi). Furthermore, therapeutic treatment with DIG and OUA inhibited over 99 % of SARS-CoV-2 replication, leading to viral inhibition at the post entry stage of the viral life cycle. Collectively, these results suggest that DIG and OUA may be an alternative treatment for COVID-19, with potential additional therapeutic effects for patients with cardiovascular disease.
- DOI: https://doi.org/https://doi.org/10.1038/s41598-020-72879-7
- ISBN or ISSN: 2045-2322
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.