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Stem Cell Research, 2020.45, 0-0, DOI: https://doi.org/10.1016/j.scr.2020.101735
Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9
Hyang-Hee Seo, Hyo-Won Han ; Jung-Hyun Kim
Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities.
- DOI: https://doi.org/10.1016/j.scr.2020.101735
- ISBN or ISSN: 1873-5061
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.