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Annals of Clinical Microbiology, 2019. 22, 9-13, DOI: https://doi.org/10.5145/ACM.2019.22.1.9
Development and Evaluation of Multiplex PCR for the Detection of Carbapenemase-Producing Enterobacteriaceae
Si Hyun Kim, Il Kwon Bae;Na Young Kim;Sae Am Song;Sunjoo Kim;Joseph Jeong;Jeong Hwan Shin
Background: The isolation of carbapenemase-producingEnterobacteriaceae (CPE) has become increasinglycommon. Continuous surveillance for these organismsis essential because their infections areclosely related to outbreaks of illness and are associatedwith high mortality rates. The aim of this studywas to develop and evaluate multiplex PCR as ameans of detecting several important CPE genessimultaneously.
Methods: We aimed to develop a multiplex PCR thatcould detect seven CPE genes simultaneously. Themultiplex PCR was composed of seven primer setsfor the detection of KPC, IMP, VIM, NDM-1, GES,OXA-23, and OXA-48. We designed different PCRproduct sizes of at least 100 bp. We evaluated theperformance of this new test using 69 CPE-positiveclinical isolates. Also, we confirmed the specificity torule out false-positive reactions by using 71 carbapenem-susceptible clinical strains.
Results: A total of 69 CPE clinical isolates showedpositive results and were correctly identified as KPC(N=14), IMP (N=13), OXA-23 (N=12), OXA-48 (N=11),VIM (N=9), GES (N=5), and NDM (N=5) by the multiplexPCR. All 71 carbapenem-susceptible clinical isolates,including Enterococcus faecalis, Escherichiacoli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, showed negativeresults.
Conclusion: This multiplex PCR can detect sevenCPE genes at a time and will be useful in clinicallaboratories. (Ann Clin Microbiol 2019;22:9-13)
- DOI: https://doi.org/10.5145/ACM.2019.22.1.9
- ISBN or ISSN: 2288-0585
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.