Journal of Pathology and Translational Medicine, 2019. 53(4), 217-224, DOI: https://doi.org/10.4132/jptm.2019.02.20

Association between Expression of 8-OHdG and Cigarette Smoking in Non-small Cell Lung Cancer

Ae Ri An, Kyoung Min Kim;Ho Sung Park;Kyu Yun Jang;Woo Sung Moon;Myoung Jae Kang;Yong Chul Lee;Jong Hun Kim;Han Jung Chae;Myoung Ja Chung

Abstract

Background: Exposure to cigarette smoking (CS) is a major risk factor for the development oflung cancer. CS is known to cause oxidative DNA damage and mutation of tumor-related genes,and these factors are involved in carcinogenesis. 8-Hydroxydeoxyguanosine (8-OHdG) is consideredto be a reliable biomarker for oxidative DNA damage. Increased levels of 8-OHdG are associatedwith a number of pathological conditions, including cancer. There are no reports on the expressionof 8-OHdG by immunohistochemistry in non-small cell lung cancer (NSCLC).

Methods:We investigated the expression of 8-OHdG and p53 in 203 NSCLC tissues using immunohistochemistryand correlated it with clinicopathological features including smoking.

Results: The expressionof 8-OHdG was observed in 83.3% of NSCLC. It was significantly correlated with a lowT category, negative lymph node status, never-smoker, and longer overall survival (p < .05) by univariateanalysis. But multivariate analysis revealed that 8-OHdG was not an independent prognosticfactor for overall survival in NSCLC patients. The aberrant expression of p53 significantlycorrelated with smoking, male, squamous cell carcinoma, and Ki-67 positivity (p < .05).

Conclusions:The expression of 8-OHdG was associated with good prognostic factors. It was positivelycorrelated with never-smokers in NSCLC, suggesting that oxidative damage of DNA cannot beexplained by smoking alone and may depend on complex control mechanisms.

• DOI: https://doi.org/10.4132/jptm.2019.02.20
• ISBN or ISSN: 2383-7837

• 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
• This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.