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Journal of Microbiology and Biotechnology, 2019. 29(12), 2006-2013, DOI: https://doi.org/10.4014/jmb.1906.06050
Improving pneumovirus isolation using a combined centrifugation and AZD1480 method
Hansaem Lee, Hye-Min Woo;Kisoon Kim; Sehee Park; Man-Seong Park; Sung Soon Kim; You-Jin Kim
The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virusparticles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitorAZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combinedmethod using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI andAZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. Thecombined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
- DOI: https://doi.org/10.4014/jmb.1906.06050
- ISBN or ISSN: 1017-7825
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.