International Journal of Stem Cells, 2019. 12(3), 484-496, DOI: https://doi.org/10.15283/ijsc19090

Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions

Jung Jin Lim, Hyung Joon Kim;Byung-ho Rhie;Man Ryul Lee;Myeong Jun Choi;Seok-Ho Hong;Kye-Seong Kim

Abstract

Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder cells, or animal products, often greatly affect the consistency of the cell culture. There are clear advantages of a defined, xeno-free, and feeder-free culture system for human pluripotent stem cells (hPSCs) cultures, since consistency in the formulations prevents lot-to-lot variability. Eliminating all non-human components reduces health risks for downstream applications, and those environments reduce potential immunological reactions from stem cells. Therefore, development of feeder-free hPSCs culture systems has been an important focus of hPSCs research. Recently, researchers have established a variety of culture systems in a defined combination, xeno-free matrix and medium that supports the growth and differentiation of hPSCs. Here we described detailed hPSCs culture methods under feeder-free and chemically defined conditions using vitronetin and TeSR-E8 medium including supplement bioactive lysophospholipid for promoting hPSCs proliferation and maintaining stemness. Keywords : Embryonic stem cells, Induced pluripotent stem cells, Feeder-free, Chemically defined conditions, Extracellular matrices

• DOI: https://doi.org/10.15283/ijsc19090
• ISBN or ISSN: 2005-3606

• 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
• This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.