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Scientific Reports, 2019. 9(1), 7536-, DOI: https://doi.org/10.1038/s41598-019-44012-w
Circulating Microbiota-Based Metagenomic Signature for Detection of Hepatocellular Carcinoma
Eun Ju Cho, Sangseob Leem;Sun Ah Kim;Jinho Yang;Yun Bin Lee;Soon Sun Kim;Jae Youn Cheong;Sung Won Cho;Ji Won Kim;Sung-Min Kim;Jung-Hwan Yoon;Taesung Park
Circulating microbial dysbiosis is associated with chronic liver disease including nonalcoholic steatohepatitis and alcoholic liver disease. In this study, we evaluated whether disease-specific alterations of circulating microbiome are present in patients with cirrhosis and hepatocellular carcinoma (HCC), and their potential as diagnostic biomarkers for HCC. We performed cross-sectional metagenomic analyses of serum samples from 79 patients with HCC, 83 with cirrhosis, and 201 matching healthy controls, and validated the results in the same number of subjects. Serum bacterial DNA was analyzed using high-throughput pyrosequencing after amplification of the V3–V4 hypervariable regions of 16S rDNA. Blood microbial diversity was significantly reduced in HCC, compared with cirrhosis and control. There were significant differences in the relative abundances of several bacterial taxa that correlate with the presence of HCC, thus defining a specific blood microbiome-derived metagenomic signature of HCC. We identified 5 microbial gene markers-based model which distinguished HCC from controls with an area under the receiver-operating curve (AUC) of 0.879 and a balanced accuracy of 81.6%. In the validation, this model accurately distinguished HCC with an AUC of 0.875 and an accuracy of 79.8%. In conclusion, circulating microbiome-based signatures may be potential biomarkers for the detection HCC.
- DOI: https://doi.org/10.1038/s41598-019-44012-w
- ISBN or ISSN: 2045-2322
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.