Stem Cell Research, 2019. 40, 101554-, DOI: https://doi.org/10.1016/j.scr.2019.101554

Generation of a NESTIN-EFGP reporter human induced pluripotent stem cell line, KSCBi005-A-1, using CRISPR/Cas9 nuclease

Youngsun Lee, Hye Young Choi; Ara Kwon; Hyeyeon Park; Mihyun Park; Yong-Ou Kim; Sungwook Kwak; Soo Kyung Koo

Abstract

NESTIN, an intermediate filament, is a neuroectodermal marker involved in induced pluripotent stem cell (iPSC) differentiation toward neural lineages. Here, we introduced an EGFP reporter into the C-terminus of NESTIN in KSCBi005-A hiPSCs through homologous recombination using CRISPR/Cas9 nuclease. The successfully edited line was confirmed by sequencing and had a normal karyotype. It expressed EGFP upon induction of neural differentiation and exhibited potential for differentiation into three germ layers. KSCBi005-A-1 cells could be used to monitor the expression of NESTIN in differentiated cell types. This cell line is available at the National Stem Cell Bank, Korea National Institute of Health.

• DOI: https://doi.org/10.1016/j.scr.2019.101554
• ISBN or ISSN: 1873-5061

• 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
• This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.