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Archives of Virology, 2019. 164(1), 127-136, DOI: https://doi.org/10.1007/s00705-018-4062-9
Development and comparison of two H5N8 influenza A vaccine candidate strains
Mi-Seon Lee, Eun Young Jang;Junhyung Cho;Kisoon Kim;Chan Hee Lee;Hwajung Yi
Avian influenza viruses circulating in birds have caused outbreaks of infection in poultry and humans, thereby threatening public health. Recently, a highly pathogenic avian influenza (HPAI) virus (H5N8) of clade 22.214.171.124 emerged in Korea and other countries and caused multiple outbreaks in domestic and wild birds, with concerns for human infection. To combat HPAI viral infections, novel vaccines are likely to be the most effective approach. Therefore, in this study, we generated H5N8 vaccine candidate viruses based on a Korean isolate (A/broiler duck/Korea/Buan2/2014). The vaccine candidate viruses were 2:6 reassortants expressing the two surface glycoproteins of A/broiler duck/Korea/Buan2/2014 on an A/Puerto Rico/8/34 (PR8) backbone generated by using an eight plasmid-based reverse genetics system with or without replacement of the multi-basic amino-acid cleavage motif (MBCM, a crucial pathogenic factor in HPAI virus) with the bi-basic amino-acid cleavage motif (BBCM) on their HA. A H5N8 vaccine candidate virus containing the BBCM showed attenuated pathogenesis in embryonated eggs and exhibited less virulence in the infected mice compared with the wild H5N8 virus containing MBCM. Vaccination with an inactivated preparation of the vaccine candidate virus protected mice from lethal H5N8 viral challenges. This is the first report of the development and evaluation of H5N8 vaccine strains (with MBCM or BBCM) of HA clade 126.96.36.199 as vaccine candidates. Our findings suggest that H5N8 strains with BBCM instead of MBCM might be considered for H5N8 vaccine seed virus development or as a reference vaccine against H5N8 viral strains.
- DOI: https://doi.org/10.1007/s00705-018-4062-9
- ISBN or ISSN: 0304-8608
- 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.